akt inhibitors miransertib arq 092  (MedChemExpress)

Journal: PLoS ONE

Article Title: Inhibitors of AKT kinase increase LDL receptor mRNA expression by two different mechanisms

doi: 10.1371/journal.pone.0218537

Figure Lengend Snippet: (A) HepG2 cells were treated with vehicle, 5 μ M AT7867, 10 μ M CCT128939, 30 μ M perifosine, 4 μ M ARQ-092, 7.5 μ M AKT inhibitor VIII, 5 μ M MK-2206 or 1 μ M triciribine for 14 h before harvesting for isolation of mRNA and determination of LDLR and GAPDH mRNA levels by qPCR assay. LDLR mRNA levels were plotted relative to the values obtained at time 0 (n = 4). (B) HepG2 cells were co-transfected with firefly luciferase reporter pLR1563-luc and Renilla luciferase plasmid. At 24 h post-transfection, cells were treated with vehicle or the indicated AKT inhibitors as in Fig 2A and then harvested for analysis of luciferase activity. After normalization of firefly luciferase activity with Renilla luciferase activity, the averages of data from four independent experiments were plotted relative to the value obtained from vehicle-treated cells that were transfected with pLR1563-luc. Error bars represent the 95% confidence interval. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with vehicle-treated cells.

Article Snippet: MK-2206 2HCl, triciribine, AT7867, ARQ-092 and CCT1298930 were obtained from Selleckchem (Houston, TX).

Techniques: Isolation, Transfection, Luciferase, Plasmid Preparation, Activity Assay